(Hydroxypropyl)methyl cellulose-chitosan film as a matrix for lipase immobilization: Operational and morphological study

The present work reports on the use of a hybrid blend biopolymers as matrix for lipase immobilization. (Hydroxypropyl)methyl cellulose (HPMC) and Chitosan (CS) were combined in order to formulate film which Mucor miehei was immobilized. biocatalyst studied upon model reaction propyl laurate synthesis. system examined terms its capability provide an appropriate environment where will maintain activity. ratio polymers used HPMC:CS=2:1 proved form most promising matrix. Increasing amount immobilized enzyme appears improve yield indicating, however, mass transfer limitations. Apparent activation energy calculated input showed that ultra-sonication accelerated initial rate reaction. Different solvents tested with isooctane being effective. enzyme-containing remarkable reusability, since it can be up 35 times without loss Finally, Atomic Force Microscopy (AFM) performed observe morphology films. HPMC/CS exhibits nanostructure unique characteristic length roughness 42.8 nm while presence smoothens decreases 5.5 nm.